Yes, you can ask your Audience Polling questions in a different order. You might start with the 4th question in your queue rather than starting with the 1st question.
Questions will continue to cycle through until all questions in the queue after that question has been asked. For example, the first question asked might be the 4th question in the queue of 6 question. Questions 4 to 6 will be asked as part of the queue, however, as questions 1 to 3 were first in the queue, these will not be asked.
Next steps: What is the Audience Polling Display Queue?